peptide mix ii Search Results


macrospin columns  (Chem Impex International)
95
Chem Impex International macrospin columns
Macrospin Columns, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/macrospin columns/product/Chem Impex International
Average 95 stars, based on 1 article reviews
macrospin columns - by Bioz Stars, 2026-03
95/100 stars
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ot1 and ot-ii peptide mix  (GenScript corporation)
90
GenScript corporation ot1 and ot-ii peptide mix
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Ot1 And Ot Ii Peptide Mix, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ot1 and ot-ii peptide mix/product/GenScript corporation
Average 90 stars, based on 1 article reviews
ot1 and ot-ii peptide mix - by Bioz Stars, 2026-03
90/100 stars
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peptide mix ii  (Bruker Corporation)
90
Bruker Corporation peptide mix ii
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Peptide Mix Ii, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peptide mix ii/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
peptide mix ii - by Bioz Stars, 2026-03
90/100 stars
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me-sims mass spectrum of the bruker peptide mix ii  (Bruker Corporation)
90
Bruker Corporation me-sims mass spectrum of the bruker peptide mix ii
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Me Sims Mass Spectrum Of The Bruker Peptide Mix Ii, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/me-sims mass spectrum of the bruker peptide mix ii/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
me-sims mass spectrum of the bruker peptide mix ii - by Bioz Stars, 2026-03
90/100 stars
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peptide standard 132 mix ii  (Bruker Corporation)
90
Bruker Corporation peptide standard 132 mix ii
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Peptide Standard 132 Mix Ii, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peptide standard 132 mix ii/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
peptide standard 132 mix ii - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
sdhb, the maldi-ms peptide calibration standard mix ii, and the protein calibration standard mix i  (Bruker Corporation)
90
Bruker Corporation sdhb, the maldi-ms peptide calibration standard mix ii, and the protein calibration standard mix i
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Sdhb, The Maldi Ms Peptide Calibration Standard Mix Ii, And The Protein Calibration Standard Mix I, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sdhb, the maldi-ms peptide calibration standard mix ii, and the protein calibration standard mix i/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
sdhb, the maldi-ms peptide calibration standard mix ii, and the protein calibration standard mix i - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
peptide standard bruker peptide mix ii  (Bruker Corporation)
90
Bruker Corporation peptide standard bruker peptide mix ii
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Peptide Standard Bruker Peptide Mix Ii, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peptide standard bruker peptide mix ii/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
peptide standard bruker peptide mix ii - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
peptide mix ii maldi standard preparation  (Bruker Corporation)
90
Bruker Corporation peptide mix ii maldi standard preparation
In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched <t>OT1</t> TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.
Peptide Mix Ii Maldi Standard Preparation, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peptide mix ii maldi standard preparation/product/Bruker Corporation
Average 90 stars, based on 1 article reviews
peptide mix ii maldi standard preparation - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched OT1 TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.

Journal: iScience

Article Title: Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture

doi: 10.1016/j.isci.2024.109006

Figure Lengend Snippet: In vitro biological characterization; ccQS-21 and beQS-21 equivalently induce hemolysis, trigger the innate immune cell stress response, and enhance antigen processing and presentation to enhance the potency of the vaccinal immune synapse (A) Upon incubation with human red blood cells both beQS-21 and ccQS-21 retain equivalent saponin-specific hemolytic properties at pH-7.4 with matching half maximal effective concentration (EC50). (B) Upon pre-conditioning of THP1 human myeloid cells with a TLR4 agonist (LPS), both beQS-21 and ccQS-21 induce inflammasome-dependent secretion of IL-1β and the production of alarmins associated with pyroptosis such as HMGB1. Both IL-1β secretion and pyroptosis can be regulated by the canonical inflammasome pathway and can be suppressed by the disruption of the genes encoding for NLRP3, ASC and Caspase-1 or by the use of Caspase-1 inhibitor, ZVAD. (C) Both beQS-21 and ccQS-21 can comparably induce a broader inflammatory response in vitro . The breadth of the inflammatory response induced by the stimulation of human granulocyte macrophage colony stimulating factor (GMCSF) and IL-4 differentiated Monocyte derived Dendritic Cells (Mo-DCs) using both QS-21 was assessed with multiplex cytokine quantification. Both beQS-21 (BE) and ccQS-21 (CC) induce the same inflammatory signature which is further enhanced by formulating with TLR4 agonist. (D) Pseudo cross-presentation assay using cell viability to demonstrate that beQS-21 and ccQS-21 equivalently induce cytotoxicity through the regulation of the cytosolic translocation from the phagolysosome (e.g., as of the indicated toxin: saporin), a critical process for antigen cross presentation. (E) Flow cytometry analysis demonstrating that both QS-21 equivalently enhance large antigen processing and cross-presentation on MHC-I using a T cell receptor (TCR)-like antibody against the H-2K b -SIINFEKL complex. (F) In vitro antigen cross-presentation assay demonstrating that ccQS-21 and beQS-21 equivalently enhance antigen processing and minimal epitope cross-presentation to activate transgenic T cells with matched OT1 TCR to express NFAT luciferase reporter. (G) OT1 T cell proliferation shows ccQS-21 and beQS-21 equivalently enhance antigen cross-presentation to induce the proliferation of primary T cells with matched OT1 TCR. OVA: Ovalbumin, OT1 long peptide: 29mer peptide including SIINFEKL, BFM: Bafilomycin A1, inhibiting the vacuolar type H + -ATPase (v-ATPase) to transfer protons into the lysosome.

Article Snippet: Cells were then plated in flat-bottom culture 96-well plates (0.5 × 10 6 cells per well). gE overlapping peptide mix (0.25 μg/mL, JPT Peptide Technologies, Germany), or OT1 and OT-II peptide mix (5 μg/mL, GenScript, China) were added for restimulation.

Techniques: In Vitro, Incubation, Concentration Assay, Disruption, Derivative Assay, Multiplex Assay, Translocation Assay, Flow Cytometry, Transgenic Assay, Luciferase

Journal: iScience

Article Title: Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture

doi: 10.1016/j.isci.2024.109006

Figure Lengend Snippet:

Article Snippet: Cells were then plated in flat-bottom culture 96-well plates (0.5 × 10 6 cells per well). gE overlapping peptide mix (0.25 μg/mL, JPT Peptide Technologies, Germany), or OT1 and OT-II peptide mix (5 μg/mL, GenScript, China) were added for restimulation.

Techniques: Recombinant, Cell Isolation, Enzyme-linked Immunospot, Cytotoxicity Assay, Luciferase, Expressing